#Flowjo change sample name manual

If you are a GLP lab, GLP guidelines suggest against this practice, since it duplicates the source data. When you choose to display the sample's file name from the Sample Name Keyword - FlowJo examines the FCS keyword header information and uses the sample name keyword from the data file as the file name in the workspace. The replace partial choice in this section means that, if checked, only the text string that you searched for will be replaced, whereas the default is to replace the entire keyword. FCS files can be exported with the new keyword labels to make the change permanent. Just below, you can choose how FlowJo will display the data file names in the workspace window list. You can refine your search using the tools in section 3.If there is something that you would like to replace the found text with, just type it into the ‘Replace with’ box in section 2 and then click either replace, or replace all.Select the file folder containing the raw data associated with your analysis. As soon as you type a value the list below (section 4) will update. Right click on a data file in that workspace and choose 'Search for FCS Files. In the area labelled 2, fill in what string of values you would like to search for.Selecting which keyword or keywords you would like to search for something in, using the drop down labelled 1 in figure 2.Therefore it may be best to do this in the workspace and save the workspace – the changes are stored in the document, which in turn links to the un-altered data.įigure 2 shows the interface that appears when the Find / Replace button is clicked. Simply select a set of populations you’d like to combine, and run Export/Concatenate. Combining populations in FlowJo is easy using concatenation. The raw FCS data files remain unchanged. FCS files can be exported with the new keyword labels to make the change permanent. By labeling samples with a numerical keyword that uniquely identifies the separate files, you can both: create a new parameter from any such keyword. Export as FCS 3.0 not FCS2 (important, since it preserves below-zero values) 3.

The changes you make with this tool affect the current workspace only. Acquire your CBA on FACSDiva as per manufacturer instructions (ie like a normal FACS expt). By default it is located in the Find band of the File ribbon as shown in Figure 1. One way to reconcile keyword value variations is through the find and replace dialog.

#Flowjo change sample name manual

Without proper labels in keywords, analysis can become error prone due to manual iteration through dozens/hundreds of samples. The Find / Replace tool is a fast approach to correcting keyword errors and omissions.įlowJo’s power truly comes down to batch analysis, and batch analysis relies heavily on keyword annotation.